fibronectin-coated 8-well labtek chamber slide Search Results


coverglass-bottomed dishes  (MatTek)
90
MatTek coverglass-bottomed dishes
Coverglass Bottomed Dishes, supplied by MatTek, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibronectin-coated+8-well+labtek+chamber+slide/glass+bottom+culture+dishes/10__1128_slash_iai__00535___13-58-15-17
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coverglass-bottomed dishes - by Bioz Stars, 2026-09
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fibronectin  (Becton Dickinson)
90
Becton Dickinson fibronectin
Biological effects induced by RANTES on EPC. (a) RANTES binds to EPC in a dose-dependent manner. Human EPC were incubated with 20 or 40 nM biotinylated RANTES (B-RANTES) and the binding were analysed by flow cytometer with avidin-FITC. Reactivity was compared to avidin-FITC alone. Data shown are representative of three independent experiments. ( b) RANTES at 3 nM induced EPC spreading on <t>fibronectin</t> layer. Results are expressed as mean ± SEM of EPC area, expressed in square-inches, measured by field for three independent experiments. * P < 0.05 versus untreated cells. (c) RANTES at 3 nM induced EPC migration in a modified Boyden chamber model. Results are expressed as mean ± SEM of EPC counted by field for three independent experiments. * P < 0.05 versus untreated cells. ( d) RANTES at 3 nM induces EPC vascular sprout area ( left panel ) and vascular sprout length ( right panel ) and in a 2D angiogenesis assay on Matrigel. The length and area of vascular sprouts formed by untreated EPC was arbitrary set to 100%. The results for cells treated with RANTES were expressed as a percentage of control cells. * P < 0.05 versus untreated cells.
Fibronectin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/fibronectin-coated+8-well+labtek+chamber+slide/fibronectin/pmc05643514-315-17-20
Average 90 stars, based on 1 article reviews
fibronectin - by Bioz Stars, 2026-09
90/100 stars
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Biological effects induced by RANTES on EPC. (a) RANTES binds to EPC in a dose-dependent manner. Human EPC were incubated with 20 or 40 nM biotinylated RANTES (B-RANTES) and the binding were analysed by flow cytometer with avidin-FITC. Reactivity was compared to avidin-FITC alone. Data shown are representative of three independent experiments. ( b) RANTES at 3 nM induced EPC spreading on fibronectin layer. Results are expressed as mean ± SEM of EPC area, expressed in square-inches, measured by field for three independent experiments. * P < 0.05 versus untreated cells. (c) RANTES at 3 nM induced EPC migration in a modified Boyden chamber model. Results are expressed as mean ± SEM of EPC counted by field for three independent experiments. * P < 0.05 versus untreated cells. ( d) RANTES at 3 nM induces EPC vascular sprout area ( left panel ) and vascular sprout length ( right panel ) and in a 2D angiogenesis assay on Matrigel. The length and area of vascular sprouts formed by untreated EPC was arbitrary set to 100%. The results for cells treated with RANTES were expressed as a percentage of control cells. * P < 0.05 versus untreated cells.

Journal: Scientific Reports

Article Title: Pro-angiogenic effect of RANTES-loaded polysaccharide-based microparticles for a mouse ischemia therapy

doi: 10.1038/s41598-017-13444-7

Figure Lengend Snippet: Biological effects induced by RANTES on EPC. (a) RANTES binds to EPC in a dose-dependent manner. Human EPC were incubated with 20 or 40 nM biotinylated RANTES (B-RANTES) and the binding were analysed by flow cytometer with avidin-FITC. Reactivity was compared to avidin-FITC alone. Data shown are representative of three independent experiments. ( b) RANTES at 3 nM induced EPC spreading on fibronectin layer. Results are expressed as mean ± SEM of EPC area, expressed in square-inches, measured by field for three independent experiments. * P < 0.05 versus untreated cells. (c) RANTES at 3 nM induced EPC migration in a modified Boyden chamber model. Results are expressed as mean ± SEM of EPC counted by field for three independent experiments. * P < 0.05 versus untreated cells. ( d) RANTES at 3 nM induces EPC vascular sprout area ( left panel ) and vascular sprout length ( right panel ) and in a 2D angiogenesis assay on Matrigel. The length and area of vascular sprouts formed by untreated EPC was arbitrary set to 100%. The results for cells treated with RANTES were expressed as a percentage of control cells. * P < 0.05 versus untreated cells.

Article Snippet: After a 24 h serum deprivation, 20 × 10 3 EPC per well were seeded on a fibronectin (100 μg/mL, BD Biosciences Pharmingen, Le Pont de Claix, France) pre-coated 8-well Labtek and incubated for 2 h at 37 °C with 3 nM RANTES to assess their spreading.

Techniques: Incubation, Binding Assay, Flow Cytometry, Avidin-Biotin Assay, Migration, Modification, Angiogenesis Assay